{"product_id":"cst-2003s","title":"CST,  2003S, BID Antibody","description":"Polyclonal Antibody for studying Bid mouse. Validated for Western Blotting. Highly specific and rigorously validated in-house, BID Antibody (CST #2003) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nBID Antibody detects endogenous levels of full length mouse BID protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site of mouse BID. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nBid is a pro-apoptotic \"BH3 domain-only\" member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax (1). Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane (2-4). Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation (5-7). Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation (8,9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2700049M22Rik; AI875481; AU022477; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BH3-interacting domain death agonist p11; BH3-interacting domain death agonist p13; BH3-interacting domain death agonist p15; BID; OTTMUSP00000028756; p11 BID; p13 BID; p15 BID; p22 BID\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 22\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46801051320489,"sku":"2003S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2003s","provider":"Iright","version":"1.0","type":"link"}