{"product_id":"cst-20055s","title":"CST,  20055S, SET1B (E7D1G) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SET1B. Validated for Western Blotting,Immunoprecipitation,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, SET1B (E7D1G) Rabbit Monoclonal Antibody (CST #20055) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nChromatin IP: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSET1B (E7D1G) Rabbit Monoclonal Antibody recognizes endogenous levels of total SET1B protein. This antibody may detect bands of unknown origin around 250, 150, and 130 kDa.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1240 of human SET1B protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Set1 histone methyltransferase protein was first identified in yeast as part of the Set1\/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, six Set1-related proteins exist in mammals: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30. These subunits are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1\/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nFLJ20803; Histone-lysine N-methyltransferase SETD1B; hSET1B; IDDSELD; KIAA1076; KMT2G; Lysine N-methyltransferase 2G; SET domain containing 1B; SET domain containing 1B, histone lysine methyltransferase; SET domain-containing protein 1B; SET1B; SETD1B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 320\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797643677865,"sku":"20055S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20055s","provider":"Iright","version":"1.0","type":"link"}