{"product_id":"cst-20083s","title":"CST,  20083S, Cas9 (7A9-3A3) Mouse Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying Cas9 bacteria. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cas9 (7A9-3A3) Mouse Monoclonal Antibody (Biotinylated) (CST #20083) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCas9 (7A9-3A3) Mouse Monoclonal Antibody (Biotinylated) recognizes transfected levels of total Cas9 protein.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant protein specific to the amino terminus of Cas9 protein. This antibody recognizes residues surrounding Arg220 of Cas9 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the CRISPR antiviral immunity system that provides adaptive immunity against extrachromosomal genetic material (1). The CRISPR antiviral mechanism of action involves three steps: (i), acquisition of foreign DNA by host bacterium; (ii), synthesis and maturation of CRISPR RNA (crRNA) followed by the formation of RNA-Cas nuclease protein complexes; and (iii), target interference through recognition of foreign DNA by the complex and its cleavage by Cas nuclease activity (2). The type II CRISPR\/Cas antiviral immunity system provides a powerful tool for precise genome editing and has potential for specific gene regulation and therapeutic applications (3). The Cas9 protein and a guide RNA consisting of a fusion between a crRNA and a trans-activating crRNA (tracrRNA) must be introduced or expressed in a cell. A 20-nucleotide sequence at the 5' end of the guide RNA directs Cas9 to a specific DNA target site. As a result, Cas9 can be \"programmed\" to cut various DNA sites both and in cells and organisms. CRISPR\/Cas9 genome editing tools have been used in many organisms, including mouse and human cells (4,5). Research studies demonstrate that CRISPR can be used to generate mutant alleles or reporter genes in rodents and primate embryonic stem cells (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\ncas9; CRISPR-associated endonuclease Cas9\/Csn1; csn1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nMW (kDa): 160\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46800357720233,"sku":"20083S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20083s","provider":"Iright","version":"1.0","type":"link"}