CST, 20087S, OTUD5 (D8Y2U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying OTUD5. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, OTUD5 (D8Y2U) Rabbit Monoclonal Antibody (CST #20087) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity OTUD5 (D8Y2U) Rabbit Monoclonal Antibody recognizes endogenous levels of total OTUD5 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OTUD5 protein. Background Protein ubiquitination and deubiquitination are reversible processes catalyzed by ubiquitinating enzymes and deubiquitinating enzymes, respectively (1,2). Deubiquitinating enzymes (DUBs) are categorized into five subfamilies based on catalytic domain structure: USP, OTU, MJD, UCH, and JAMM/MPN. OTUD5, also known as DUBA, belongs to the OTU family of DUBs. Phosphorylation of OTUD5 at Ser177 is required to activate its DUB activity (3). Research studies have shown that OTUD5 plays a pivotal role in negatively regulating innate immune responses though its ability to bind and deubiquitinate K63-linked TRAF3, thereby suppressing production of IFN-I by macrophages (4). There is also evidence that OTUD5 participates in the adaptive immune response by negatively regulating the production of pro-inflammatory IL-17 by Th17 cells (5). Alternate Names deubiquinating enzyme A; Deubiquitinating enzyme A; DKFZp761A052; DUBA; MGC104871; OTU deubiquitinase 5; OTU domain containing 5; OTU domain-containing protein 5; OTUD5 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 75 Source/Isotype: Rabbit IgG