CST, 20272S, IRP1 (D6S4J) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IRP1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IRP1 (D6S4J) Rabbit Monoclonal Antibody (CST #20272) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IRP1 (D6S4J) Rabbit Monoclonal Antibody recognizes endogenous levels of total IRP1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu734 of human IRP1 protein. Background Iron regulatory proteins (IRPs; also known as IREBs) are RNA-binding proteins that recognize iron-responsive elements (IREs) and play an important role in maintaining iron homeostasis in mammalian cells. IREs are conserved cis-regulatory hairpin structures located within the 5' or 3' untranslated regions (UTRs) of target mRNAs. IRPs inhibit translation when bound to IREs within the 5' UTR of mRNA encoding for proteins involved in iron storage, export, and utilization. IRP binding to multiple IREs within the 3' UTR of transferin receptor 1 (TFR1) mRNA prevents its degradation, thereby augmenting translation of TFR1 and increasing iron uptake into cells (1-3). Dysregulation of IRPs has been associated with human cancers (4-6). IRP1 is a bifunctional protein. In iron replete cells, IRP1 is associated with a 4Fe-4S cluster that keeps IRP1 in a closed conformation, and functions as a cytosolic aconitase catalyzing the conversion of citrate into iso-citrate. In iron deficient cells, IRP1 loses the 4Fe-4S cluster and adopts an open structure with IRE-binding activity (7). Alternate Names ACO1; ACOC; Aconitase; aconitase 1; aconitase 1, soluble; aconitate hydratase; aconitate hydratase, cytoplasmic; ACONS; Citrate hydro-lyase; cytoplasmic aconitase; Cytoplasmic aconitate hydratase; cytosplasmic aconitase; epididymis luminal protein 60; epididymis secretory sperm binding protein; Ferritin repressor protein; HEL60; IRE-BP 1; IREB1; IREBP; IREBP1; Iron regulatory protein 1; iron-responsive element binding protein 1; Iron-responsive element-binding protein 1; IRP1; soluble aconitase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 98 Source/Isotype: Rabbit IgG