{"product_id":"cst-20301t","title":"CST,  20301T, SRC-1 (D1M3Y) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SRC-1. Validated for Western Blotting,Chromatin IP,CUT\u0026amp;RUN. Available in 2 sizes. Highly specific and rigorously validated in-house, SRC-1 (D1M3Y) Rabbit Monoclonal Antibody (CST #20301) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nChromatin IP: 1:50\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Chromatin IP, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSRC-1 (D1M3Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total SRC-1 protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala12 of human SRC-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThere are three members of the steroid receptor co-activator (SRC) family of proteins: SRC-1 (NCoA-1), SRC-2 (TIF2\/GRIP1\/NCoA-2), and SRC-3 (ACTR\/pCIP\/RAC3\/TRAM-1\/AIB1). All SRC family members share significant structural homology and function to stimulate transcription mediated by nuclear hormone receptors and other transcriptional activators such as Stat3, NF-κB, E2F1, and p53 (1-4). Two SRC proteins, SRC-1 and SRC-3, function as histone acetyltransferases (5,6). In addition, all three family members can recruit other histone acetyltransferases (CBP\/p300, PCAF) and histone methyltransferases (PRMT1, CARM1) to target promoters and cooperate to enhance expression of many genes (5-8). The SRC proteins play important roles in multiple physiological processes including cell proliferation, cell survival, somatic cell growth, mammary gland development, female reproductive function, and vasoprotection (9). SRC-1 and SRC-3 are conduits for kinase-mediated growth factor signaling to the estrogen receptor and other transcriptional activators. Seven SRC-1 phosphorylation sites and six SRC-3 phosphorylation sites have been identified, which are induced by steroids, cytokines, and growth factors and involve multiple kinase signaling pathways (9-11). Research has shown that all three SRC family members are associated with increased activity of nuclear receptors in breast, prostate, and ovarian carcinomas. According to the literature, SRC-3 is frequently amplified or overexpressed in a number of cancers (12), and SRC-1\/PAX3 and SRC-2\/MYST3 translocations are found associated with rhabdomyosarcoma and acute myeloid leukemia, respectively (13,14).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nbHLHe42; BHLHE74; Class E basic helix-loop-helix protein 74; F-SRC-1; Hin-2 protein; KAT13A; MGC129719; MGC129720; NCoA-1; NCOA1; Nuclear receptor coactivator 1; PAX3\/NCOA1 fusion protein; Protein Hin-2; Renal carcinoma antigen NY-REN-52; RIP160; SRC-1; SRC1; Steroid receptor coactivator 1; steroid receptor coactivator-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 180\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797650198697,"sku":"20301T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20301t","provider":"Iright","version":"1.0","type":"link"}