CST, 20312S, Fc-gamma RIIB (D8T4C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FcgR2b. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Fc-gamma RIIB (D8T4C) Rabbit Monoclonal Antibody (CST #20312) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Fc-gamma RIIB (D8T4C) Rabbit Monoclonal Antibody recognizes endogenous levels of total FcγRIIB protein. Based on western blot analysis of lysates from cells transfected with constructs expressing FcγRIIA or FcγRIIB, this antibody has a strong preference for FcγRIIB and demonstrates weak cross-reactivity with FcγRIIA. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human FcγRIIB protein. Background FcγRIIB (CD32B) is a low affinity, IgG Fc-binding receptor expressed on B cells, monocytes, macrophages, and dendritic cells (DCs) (1-3). It is the inhibitory Fc receptor and signals through an immunoreceptor tyrosine-based inhibitory motif (ITIM) within its carboxy-terminal cytoplasmic tail (2). Binding of immune complexes to FcγRIIB results in tyrosine phosphorylation of the ITIM motif at Tyr292 and recruitment of the phosphatase SHIP, which mediates inhibitory effects on immune cell activation (2,4). In this way, FcγRIIB suppresses the effects of activating Fc-binding receptors (3). For example, mice deficient for FcγRIIB have greater T cell and DC responses following injection of immune complexes (5,6). In addition, FcγRIIB plays a role in B cell affinity maturation (7). Signaling through FcγRIIB in the absence of signaling through the B cell receptor (BCR) is proapoptotic, while signaling through FcγRIIB and the BCR simultaneously attenuates the apoptotic signal and results in selection of B cells with higher antigen affinity (7). Alternate Names CD32; CD32B; CDw32; Fc fragment of IgG receptor IIb; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma receptor IIb; Fc gamma RIIb; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma-RIIb; Fc-gamma-RIIc; FCG2; FCG2B; FCGR2; FCGR2B; FCGR2C; FcRII-b; FcRII-c; IGFR2; IgG Fc receptor II-b; IgG Fc receptor II-c; Low affinity immunoglobulin gamma Fc region receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II-c Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-50 Source/Isotype: Rabbit IgG