CST, 2032S, Lamin A/C Antibody

Polyclonal Antibody for studying Lamin A/C (full-length). Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Lamin A/C Antibody (CST #2032) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:300 - 1:1200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Lamin A/C Antibody detects endogenous levels of total full length lamin A (and lamin C) (70 kDa), as well as the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230 (28 kDa). Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp230 of human lamin A. Antibodies are purified by protein A and peptide affinity chromatography. Background Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6). Alternate Names 70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A/C; lamin A/C-like 1; Lamin-A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; Prelamin-A/C; PRO1; Renal carcinoma antigen NY-REN-32 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28, 70 SOURCE: Rabbit