CST, 2036T, Cleaved Lamin A (Small Subunit) (30H5) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Lamin A/C (small subunit) cleaved. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Lamin A (Small Subunit) (30H5) Mouse Monoclonal Antibody (CST #2036) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Cleaved Lamin A (Small Subunit) (30H5) Mouse Monoclonal Antibody detects endogenous levels of the small fragment of lamin A (and lamin C) resulting from cleavage at aspartic acid 230. This antibody does not cross-react with full length lamin A or C. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp230 of human lamin A. Background Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into a large (41-50 kDa) and a small (28 kDa) fragment (3,4). The cleavage of lamins results in nuclear dysregulation and cell death (5,6). Alternate Names 70 kDa lamin; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; epididymis secretory sperm binding protein; FPL; FPLD; FPLD2; HGPS; IDC; lamin; lamin A/C; lamin A/C-like 1; Lamin-A/C; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1; MADA; mandibuloacral dysplasia type A; Prelamin-A/C; PRO1; Renal carcinoma antigen NY-REN-32 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 28 Source/Isotype: Mouse IgG1