CST, 20407S, SerpinB1 (E4W7B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SERPINB1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, SerpinB1 (E4W7B) Rabbit Monoclonal Antibody (CST #20407) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 73485 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity SerpinB1 (E4W7B) Rabbit Monoclonal Antibody recognizes endogenous levels of total SerpinB1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu264 of human SerpinB1 protein. Background Serpin family B member 1 (SerpinB1), also known as leukocyte elastase inhibitor or neutrophil elastase inhibitor, regulates inflammation and the innate immune response. SerpinB1 blocks the activity of caspase-1, caspase-4, and caspase-5; the protein does so via suppression of caspase recruitment domain oligomerization and enzymatic activation (1). SerpinB1 is expressed in neutrophils and monocytes, and the protein resides in the cytoplasm due to its lack of a secretory signal sequence (2,3). There, SerpinB1 protects cells from serine proteases that may leak from granules into the cytoplasm, including Cathepsin G, neutrophil elastase, proteinase-3, and Granzyme H (4,5). Despite its lack of a secretory sequence, SerpinB1 was shown to be released from keratinocytes in a caspase-1-dependent manner to upregulate proliferation of pancreatic β cells (6). Alternate Names anti-elastase; EI; ELANH2; epididymis luminal protein 57; epididymis secretory protein Li 27; HEL-S-27; HEL57; ILEU; LEI; Leukocyte elastase inhibitor; M/NEI; MNEI; Monocyte/neutrophil elastase inhibitor; Peptidase inhibitor 2; PI-2; PI2; protease inhibitor 2 (anti-elastase), monocyte/neutrophil derived; serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 1; Serpin B1; serpin family B member 1; serpin peptidase inhibitor, clade B (ovalbumin), member 1; SERPINB1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG