CST, 20473T, Phospho-Tau (Thr231/Ser235) (E7G5W) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tau iso8 (Thr231/Ser235) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Tau (Thr231/Ser235) (E7G5W) Rabbit Monoclonal Antibody (CST #20473) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-Tau (Thr231/Ser235) (E7G5W) Rabbit Monoclonal Antibody recognizes endogenous levels of tau protein only when phosphorylated at Thr231 and Thr231/Ser235. This antibody does not detect phosphorylation at Ser235 alone. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr231 of human tau protein. Background Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3). It has been shown that phosphorylation of tau at Thr231/Ser235 is elevated in AD brain and human tau-transgenic mouse models of AD, where it may be involved in increasing tau seeding efficiency (4,5). Tau phosphorylation at Thr231 can be detected in cerebrospinal fluid and plasma and shows potential as a biomarker of neurofibrillary pathology in AD (6,7). Alternate Names DDPAC; FLJ31424; FTDP-17; G protein beta1/gamma2 subunit-interacting factor 1; MAPT; MAPTL; MGC138549; microtubule associated protein tau; Microtubule-associated protein tau; MSTD; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; PPND; PPP1R103; protein phosphatase 1, regulatory subunit 103; TAU; Tau iso8 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 55-80 Source/Isotype: Rabbit IgG