CST, 20483S, Desmoglein 3 (5G11) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Desmoglein-3. Validated for Western Blotting,Simple Western™,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Desmoglein 3 (5G11) Mouse Monoclonal Antibody (CST #20483) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Desmoglein 3 (5G11) Mouse Monoclonal Antibody recognizes endogenous levels of total desmoglein 3 protein. This antibody does not cross-react with desmoglein 1 or desmoglein 2 proteins. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the sequence of human desmoglein 3 protein. Background Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, which are intercellular junctions that mediate strong cell-cell adhesion and are linked to the intermediate filament cytoskeleton. Dysregulation of desmosomal protein expression affects both cell adhesion and communication, and has been shown to result in various human pathologies (1). Desmoglein 3 (DSG3) expression is altered in some human cancers, and its expression may be linked to tumor progression (2). Auto-antibodies against desmoglein 1 and desmoglein 3 cause Pemphigus, a life-threatening autoimmune disorder that results in blistering of the skin and mucous membranes (3). Alternate Names 130 kDa pemphigus vulgaris antigen; 130-kD pemphigus vulgaris antigen; Cadherin family member 6; CDHF6; desmoglein 3; desmoglein 3 (pemphigus vulgaris antigen); Desmoglein-3; DKFZp686P23184; DSG3; pemphigus vulgaris antigen; PVA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 130 Source/Isotype: Mouse IgG1 kappa