CST, 2049S, Tug Antibody

Polyclonal Antibody for studying TUG. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Tug Antibody (CST #2049) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Tug Antibody detects endogenous levels of total Tug protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around residue Val542 of human Tug. Antibodies are purified by Protein A and peptide affinity chromatography. Background Tug (Tether containing UBX domain for GLUT4), also known as ASPL, ASPSCR1, RCC17, UBXD9, UBXN9, was first identified as a chromosomal translocation partner for TFE3 in patients with Alveolar soft part sarcoma (1) and contains an UBX-like domain in its C-terminal region. Tug is found to tether GLUT4 in intracellular vesicles and to release GLUT4 for cell surface translocation upon insulin stimulation (2). Stable Tug depletion or expression of a dominant negative form stimulates GLUT4 redistribution (3). Alternate Names Alveolar soft part sarcoma chromosomal region candidate gene 1 protein; alveolar soft part sarcoma chromosome region, candidate 1; Alveolar soft part sarcoma locus; ASPC1; ASPCR1; ASPL; ASPS; ASPSCR1; ASPSCR1 tether for SLC2A4, UBX domain containing; ASPSCR1, UBX domain containing tether for SLC2A4; FLJ45380; RCC17; renal cell carcinoma gene on chromosome 17; renal cell carcinoma, papillary, 17; Renal papillary cell carcinoma protein 17; Tether containing UBX domain for GLUT4; TUG; UBX domain protein 9; UBX domain-containing protein 9; UBXD9; UBXN9 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70-75 SOURCE: Rabbit