{"product_id":"cst-20591sf","title":"CST,  20591SF, Fibrillarin (C13C3) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying Fibrillarin. Validated for Western Blotting,Immunofluorescence (Frozen),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Fibrillarin (C13C3) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #20591) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #2639. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 2639\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFibrillarin (C13C3) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total fibrillarin protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Thr298 of human fibrillarin.\n\u003cb\u003eBackground\u003c\/b\u003e\nFibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place (1,2). Fibrillarin associates with several other structural proteins as well as box C\/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence (3,4). The sequence, structure, and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FBL; FBRL; FIB; FIB1; fibrillarin; FLRN; Histone-glutamine methyltransferase; Nop1; RNA, U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2'-O-methyltransferase fibrillarin; U6 snRNA 2'-O-methyltransferase fibrillarin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 37\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797652361385,"sku":"20591SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20591sf","provider":"Iright","version":"1.0","type":"link"}