CST, 20681S, Sortilin Antibody

Polyclonal Antibody for studying Sortilin. Validated for Western Blotting. Highly specific and rigorously validated in-house, Sortilin Antibody (CST #20681) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Sortilin Antibody recognizes endogenous levels of total sortilin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val361 of human sortilin protein. Antibodies are purified by peptide affinity chromatography. Background Sortilin is a multifunctional member of the vacuolar protein sortin-10 (VPS10) receptor family containing type-1 transmembrane proteins that have pleiotropic function in neuronal protein trafficking and viability (1). Sortilin is implicated to have inhibitory effects on tau aggregation in Alzheimer's disease transgenic murine models expressing human mutated tau P301S (2), and also supports to regulate intracellular processing of amyloid precursor proteins (APP) (3,4) and β-secretase, which cleaves APP to produce neurotoxic Aβ peptides (5). Sortilin also plays a role in frontotemporal lobar degeneration (FTLD) by binding and regulating extracellular concentrations of the secreted glycoprotein progranulin (6). Sortilin has been linked to cholesterol circulating concentration and lifetime risk of developing significant atherosclerotic disease. Higher concentrations of sortilin have been found in patients with coronary atherosclerosis (7). Sortilin functions in the immune system within microglia, pro-inflammatory cytokine regulation, phagosome fusion, and pathogen clearance (8). Alternate Names 100 kDa NT receptor; Glycoprotein 95; Gp95; LDLCQ6; Neurotensin receptor 3; NT3; NTR3; SORT; SORT1; Sortilin; sortilin 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit