CST, 20729S, MATK/CHK (D2I6U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CTK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MATK/CHK (D2I6U) Rabbit Monoclonal Antibody (CST #20729) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MATK/CHK (D2I6U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MATK/CHK protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu97 of human MATK/CHK protein. Background MATK/CHK (CTK, NTK and HYL) is a non-receptor tyrosine kinase structually and functionally homologous to Csk kinase. The kinase was identified through molecular cloning from multiple tissues by different research groups. Like Csk, MATK/CHK has a N-terminal SH3 domain, followed by an SH2 domain and a C-terminal catalytic kinase domain (1-4). MATK/CHK inhibits Src family members in several different ways. First, it directly phosphorylates the inhibitory C-terminal tyrosine of Src (as well as other Src family members). This induces a Src protein conformational change from the active to inactive state (2,4). Second, it binds directly to activated Src and induces a conformational change to the inactive state (5,6). The SH2 domain of MATK/CHK directly interacts with the phosphorylated tyrosine of activated receptor tyrosine kinases, such as ErbB-2 and c-Kit, to inhibit receptor function (7-9). MATK/CHK negatively regulates tumor cell growth, migration and invasion (10-13). Decreased expression of the protein has been correlated with brain tumors as well as colon cancers in research studies (14-15). Alternate Names CHK; CSK homologous kinase; Csk-homologous kinase; Csk-type protein tyrosine kinase; CTK; DKFZp434N1212; Hematopoietic consensus tyrosine-lacking kinase; HHYLTK; hydroxyaryl-protein kinase; HYL; HYL tyrosine kinase; HYLTK; leukocyte carboxyl-terminal src kinase related; Lsk; MATK; megakaryocyte-associated tyrosine kinase; Megakaryocyte-associated tyrosine-protein kinase; MGC1708; MGC2101; Protein kinase HYL; tyrosine kinase MATK; Tyrosine-protein kinase CTK; tyrosylprotein kinase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 52,56 Source/Isotype: Rabbit IgG