{"product_id":"cst-20729t","title":"CST,  20729T, MATK\/CHK (D2I6U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CTK. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MATK\/CHK (D2I6U) Rabbit Monoclonal Antibody (CST #20729) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMATK\/CHK (D2I6U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MATK\/CHK protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu97 of human MATK\/CHK protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nMATK\/CHK (CTK, NTK and HYL) is a non-receptor tyrosine kinase structually and functionally homologous to Csk kinase. The kinase was identified through molecular cloning from multiple tissues by different research groups. Like Csk, MATK\/CHK has a N-terminal SH3 domain, followed by an SH2 domain and a C-terminal catalytic kinase domain (1-4). MATK\/CHK inhibits Src family members in several different ways. First, it directly phosphorylates the inhibitory C-terminal tyrosine of Src (as well as other Src family members). This induces a Src protein conformational change from the active to inactive state (2,4). Second, it binds directly to activated Src and induces a conformational change to the inactive state (5,6). The SH2 domain of MATK\/CHK directly interacts with the phosphorylated tyrosine of activated receptor tyrosine kinases, such as ErbB-2 and c-Kit, to inhibit receptor function (7-9). MATK\/CHK negatively regulates tumor cell growth, migration and invasion (10-13). Decreased expression of the protein has been correlated with brain tumors as well as colon cancers in research studies (14-15).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCHK; CSK homologous kinase; Csk-homologous kinase; Csk-type protein tyrosine kinase; CTK; DKFZp434N1212; Hematopoietic consensus tyrosine-lacking kinase; HHYLTK; hydroxyaryl-protein kinase; HYL; HYL tyrosine kinase; HYLTK; leukocyte carboxyl-terminal src kinase related; Lsk; MATK; megakaryocyte-associated tyrosine kinase; Megakaryocyte-associated tyrosine-protein kinase; MGC1708; MGC2101; Protein kinase HYL; tyrosine kinase MATK; Tyrosine-protein kinase CTK; tyrosylprotein kinase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 52,56\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797653115049,"sku":"20729T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20729t","provider":"Iright","version":"1.0","type":"link"}