{"product_id":"cst-2073s","title":"CST,  2073S, Naked2 (C67C4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Naked2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Naked2 (C67C4) Rabbit Monoclonal Antibody (CST #2073) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNaked2 (C67C4) Rabbit Monoclonal Antibody detects endogenous level of Naked2 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala217 of human Naked2.\n\u003cb\u003eBackground\u003c\/b\u003e\nNaked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt\/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt\/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt\/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDvl-binding protein NKD2; hNkd2; naked cuticle homolog 2; naked cuticle homolog 2 (Drosophila); naked cuticle-2; Naked-2; Naked2; NKD inhibitor of WNT signaling pathway 2; NKD2; NKD2, WNT signaling pathway inhibitor; Protein naked cuticle homolog 2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 59, 61\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797653246121,"sku":"2073S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2073s","provider":"Iright","version":"1.0","type":"link"}