{"product_id":"cst-20750t","title":"CST,  20750T, Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Casp9 (Asp315) cleaved. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Cleaved Caspase-9 (Asp315) (D8I9E) Rabbit Monoclonal Antibody (CST #20750) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 24731 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCleaved Caspase-9 (Asp315) (D8I9E) Rabbit Monoclonal Antibody recognizes endogenous levels of caspase-9 protein only when cleaved at Asp315. Non-specific proteins that are induced by apoptosis under certain conditions may be detected.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9\/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAPAF-3; APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP-9; CASP9; caspase 9; caspase 9, apoptosis-related cysteine peptidase; caspase 9, apoptosis-related cysteine protease; Caspase-9; Caspase-9 subunit p10; Caspase-9 subunit p35; CASPASE-9c; ICE-LAP6; ICE-like apoptotic protease 6; MCH6; PPP1R56; protein phosphatase 1, regulatory subunit 56\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 35\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797653541033,"sku":"20750T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20750t","provider":"Iright","version":"1.0","type":"link"}