CST, 20752SF, OLR1/LOX1 (E9C5A) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying OLR1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, OLR1/LOX1 (E9C5A) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #20752) is ready to ship. Product Usage Information This product is the carrier free version of product #57946. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 57946 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity OLR1/LOX1 (E9C5A) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total OLR1/LOX1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val230 of human OLR1/LOX1 protein. Background Oxidatively modified low-density lipoprotein (Ox-LDL) causes endothelial cell dysfunction and activation and contributes to atherosclerosis. Oxidized low-density lipoprotein receptor 1 (OLR1), also known as lectin-like oxidized LDL receptor 1 (LOX1), was cloned and identified as a cell membrane receptor for Ox-LDL in vascular endothelial cells (1). In addition, research studies show that is one of the most overexpressed genes in human polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) that regulate immune responses in cancer. OLR1/LOX1 is a specific marker of human PMN-MDSC (2). Furthermore, OLR1/LOX1 was shown to be upregulated in esophageal cancer. OLR1/LOX1 deficiency stimulates autophagic cell death, inhibiting esophageal cancer development (3). Alternate Names C-type lectin domain family 8 member A; CLEC8A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX-1; LOX1; LOXIN; OLR1; ox LDL receptor 1; Ox-LDL receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein receptor 1; Oxidized low-density lipoprotein receptor 1; Oxidized low-density lipoprotein receptor 1, soluble form; SCARE1; scavenger receptor class E, member 1; SLOX1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35-42 Source/Isotype: Rabbit IgG