{"product_id":"cst-2079s","title":"CST,  2079S, Miwi (G82) Antibody","description":"Polyclonal Antibody for studying Miwi. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Miwi (G82) Antibody (CST #2079) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Frozen): 1:200 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nMiwi (G82) Antibody detects endogenous levels of total Miwi protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Gly82 of mouse Miwi. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including , and (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCT80.1; HIWI; MIWI; PIWI; piwi homolog; piwi like RNA-mediated gene silencing 1; piwi-like 1 (Drosophila); Piwi-like protein 1; PIWIL1; PIWL1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 90\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797653967017,"sku":"2079S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2079s","provider":"Iright","version":"1.0","type":"link"}