{"product_id":"cst-20808t","title":"CST,  20808T, Cyclin E1 (D7T3U) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Cyclin E1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin E1 (D7T3U) Rabbit Monoclonal Antibody (CST #20808) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 83559 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCyclin E1 (D7T3U) Rabbit Monoclonal Antibody recognizes endogenous levels of total cyclin E1 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect all isoforms of cyclin E1. This antibody does not cross-react with cyclin E2.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly351 of human cyclin E1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation\/activation of the CDK inhibitors p21 Waf1\/Cip1 and p27 Kip1 prevent cyclin E\/CDK2 activation, resulting in G1\/S arrest. When conditions are favorable for cell cycle progression, cyclin D\/CDK4\/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1\/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E\/CDK2 (1,2). Cyclin E\/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E\/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites including Thr62, Ser88, Ser72, Thr380, and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1\/S-specific cyclin-E1; pCCNE1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797654098089,"sku":"20808T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20808t","provider":"Iright","version":"1.0","type":"link"}