{"product_id":"cst-20822w2","title":"CST,  20822W2, PCNA (PC10) Mouse Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate)","description":"Monoclonal Antibody for studying PCNA. Highly specific and rigorously validated in-house, PCNA (PC10) Mouse Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 750 Conjugate) (CST #20822) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPCNA (PC10) Mouse mAb (SignalFlex™ Alexa Fluor\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Bovine, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a recombinant Protein A-PCNA fusion protein obtained from pC2T.\n\u003cb\u003eBackground\u003c\/b\u003e\nProliferating cell nuclear antigen (PCNA) is a member of the DNA sliding clamp family of proteins that assist in DNA replication (1). Crystal structure data suggests that a PCNA homotrimer ring can encircle and slide along the DNA double helix (2). Multiple proteins involved in DNA replication, DNA repair, and cell cycle control bind to PCNA rather than directly associating with DNA, thus facilitating fast processing of DNA (reviewed in 3). PCNA protein expression is a well-accepted marker of proliferation.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATLD2; Cyclin; DNA polymerase delta auxiliary protein; MGC8367; PCNA; Proliferating cell nuclear antigen\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk B Pg\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800359456937,"sku":"20822W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20822w2","provider":"Iright","version":"1.0","type":"link"}