CST, 20843S, Asparagine Synthetase Antibody

Polyclonal Antibody for studying ASNS. Validated for Western Blotting. Highly specific and rigorously validated in-house, Asparagine Synthetase Antibody (CST #20843) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Asparagine Synthetase Antibody recognizes endogenous levels of total asparagine synthetase protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human asparagine synthetase protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Asparagine synthetase (ASNS) catalyzes the synthesis of asparagine from aspartate and glutamine. Research studies have shown that intracellular asparagine can suppress apoptosis in a large number of human tumors. In addition, ASNS expression levels have been associated with the progression of gliomas and neuroblastomas in patients (1). Furthermore, acute lymphocytic leukemia cells frequently depend upon serum asparagine for their viability, as they lack ASNS (2). Deprivation of asparagine by L-asparaginase has therefore been developed as a therapeutic treatment for acute lymphocytic leukemia (2,3). In subsets of gastric and hepatic cancers, ASNS promoter hypermethylation correlates with low ASNS expression, sensitizing these cancers to the asparaginase treatment (4). Alternate Names ASNS; ASNSD; asparagine synthetase; asparagine synthetase (glutamine-hydrolyzing); Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; TS11; TS11 cell cycle control protein Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 64 SOURCE: Rabbit