{"product_id":"cst-20888s","title":"CST,  20888S, TIA-1 (F3C8T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying TIA1 mouse. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, TIA-1 (F3C8T) Rabbit Monoclonal Antibody (CST #20888) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nTIA-1 (F3C8T) Rabbit mAb recognizes endogenous levels of total TIA-1 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse TIA-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nT-cell intracellular antibody 1 (TIA-1) is a member of the RNA-recognition motif (RRM) family of RNA-binding proteins that was originally found to induce DNA fragmentation in digitonin-permeabilized thymocytes (1). TIA-1 protein has about 80% identity to the related TIAR protein, both of which possess three amino-terminal RRM domains and a glutamine-rich carboxyl terminus (1,2). Alternative splicing is responsible for generating at least two isoforms of TIA-1 and TIAR (3,4). Several research studies indicate that TIA-1 and TIAR play a role in apoptosis, cellular stress, and inflammation. Importantly, TIA-1 and TIAR translocate from the nucleus to stress granules in response to a variety of environmental stresses (5-8). Stress granules function as sites of translational repression in response to potentially damaging conditions. mRNA transcripts targeted by TIA-1 and TIAR include TNF-α, Cox2, cytochrome c, GADD45α, and HIF-1α (8-13).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2310050N03Rik; AI256674; Cytotoxic granule associated RNA binding protein TIA1; cytotoxic granule-associated RNA binding protein 1; cytotoxic granule-associated RNA-binding protein 1; mTIA; mTIA-1; Nucleolysin TIA-1; OTTMUSP00000026222; RNA-binding protein TIA-1; T-cell-restricted intracellular antigen-1; Tia; TIA-1; Tia1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 42, 44\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46801052074153,"sku":"20888S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20888s","provider":"Iright","version":"1.0","type":"link"}