CST, 2089S, MCF2/Dbl Antibody

Polyclonal Antibody for studying MCF2. Validated for Western Blotting. Highly specific and rigorously validated in-house, MCF2/Dbl Antibody (CST #2089) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity MCF2/Dbl Antibody recognizes endogenous levels of total MCF2/Dbl protein. Based on amino acid sequence homology, this antibody is expected to recognize splice variants 1-4 of human MCF2/Dbl. The antibody is not expected to recognize the onco-Dbl protein formed through amino-terminal truncation. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human MCF2/Dbl. Antibodies are purified using protein A and peptide affinity chromatography. Background The MCF2/Dbl proto-oncogene product is the founding member of the Dbl family of Rho guanine nucleotide exchange factors (GEFs) that are characterized by their Dbl homology (DH) domain (1). GEFs stimulate the formation of the active, GTP-bound form of small GTPases such as Rho, Rac and Cdc42, signaling to various downstream molecules and regulating diverse cell functions. While the overexpressed, full-length Dbl gene has transforming activity (2), mutations resulting in truncated Dbl cause the protein to become highly oncogenic. This truncated form of Dbl, which lacks the amino-terminal 497 amino acids, has constitutive GEF activity (3) and is more stable than the full-length variant (4), allowing for increased signaling to downstream effector molecules. Dbl interacts with ezrin, a member of the ezrin/radixin/moesin (ERM) family of proteins that links the plasma membrane to the actin cytoskeleton. Dbl interacts with ezrin in lipid microdomains, which leads to Cdc42 activation and the regulation of processes such as filopodia formation and cell polarity (5,6). Dbl localization and biological activities are regulated in part by phosphatidylinositol 3-kinase (PI3K) (7). Dbl is also involved in cell survival and inhibits apoptosis through induction of Akt phosphorylation at Thr308 (8). Alternate Names ARHGEF21; DBL; DBL-transforming protein; MCF.2 cell line derived transforming sequence; MCF2; MCF2-transforming protein; Oncogene MCF2 (oncogene DBL); Proto-oncogene DBL; Proto-oncogene MCF-2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit