{"product_id":"cst-20905s","title":"CST,  20905S, Total Cyclin E1 Matched Antibody Pair","description":"Matched Antibody Pair for studying Cyclin E1 in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nMatched Antibody Pairs consist of capture and detection antibodies that bind to non-overlapping epitopes. For specific identification of the capture and detection antibodies in this pair, please refer to the data figure caption. Optimal dilutions\/concentrations should be determined by the end user.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20ºC. This product will freeze at -20ºC so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles . A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation\/activation of the CDK inhibitors p21 Waf1\/Cip1 and p27 Kip1 prevent cyclin E\/CDK2 activation, resulting in G1\/S arrest. When conditions are favorable for cell cycle progression, cyclin D\/CDK4\/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1\/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E\/CDK2 (1,2). Cyclin E\/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E\/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites including Thr62, Ser88, Ser72, Thr380, and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1\/S-specific cyclin-E1; pCCNE1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H","brand":"CST","offers":[{"title":"Default Title","offer_id":46800305914025,"sku":"20905S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-20905s","provider":"Iright","version":"1.0","type":"link"}