CST, 2090S, CDK7 Antibody

Polyclonal Antibody for studying Cdk7. Validated for Western Blotting. Highly specific and rigorously validated in-house, CDK7 Antibody (CST #2090) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CDK7 Antibody detects endogenous levels of total CDK7 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human CDK7. Antibodies are purified using protein A and peptide affinity chromatography. Background CDK-activating kinase (CAK) is a complex of CDK7 and cyclin H. The complex is involved in cell cycle regulation by phosphorylating an activating residue in the T-loop domain of cdks (1). Regulation of CAK activity is mediated by T-loop phosphorylation and by association with MAT1, both of which enhance its kinase activity toward the CTD of RNA polymerase II (2,3) and other substrates such as p53 (4). CAK is an essential component of the transcription complex TFIIH and may interact directly with TFIIH helicases (5). Alternate Names 39 kDa protein kinase; CAK; CAK1; CDK-activating kinase; CDK-activating kinase 1; CDK7; CDKN7; Cell division protein kinase 7; cyclin dependent kinase 7; Cyclin-dependent kinase 7; cyclin-dependent kinase 7 (MO15 homolog, Xenopus laevis, cdk-activating kinase); HCAK; homolog of Xenopus MO15 Cdk-activating kinase; kinase subunit of CAK; MO15; p39 Mo15; p39MO15; serine/threonine kinase stk1; serine/threonine protein kinase 1; serine/threonine protein kinase MO15; Serine/threonine-protein kinase 1; STK1; TFIIH basal transcription factor complex kinase subunit Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit