CST, 2093S, SCF (C19H6) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SCF. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, SCF (C19H6) Rabbit Monoclonal Antibody (CST #2093) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity SCF (C19H6) Rabbit Monoclonal Antibody detects endogenous levels of SCF by immunohistochemical staining and by flow cytometry. Western blotting detects recombinant SCF. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids in the N-terminal sequence of human SCF. Background Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis and fertility. SCF is also known as mast cell growth factor (MCGF), steel factor (SLF), or kit ligand (KL) (1-3). SCF mediates its biological effects by binding to and activating c-Kit (4). SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways (5,6). SCF/c-Kit may take part in the growth control of human malignancies (7). Alternate Names c-Kit ligand; DCUA; DFNA69; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; FPHH; Kit ligand; Kitl; KITLG; KL-1; Mast cell growth factor; MGF; SCF; SF; SHEP7; sKITLG; SLF; Soluble KIT ligand; steel factor; Stem cell factor Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG