CST, 2098S, RhoB Antibody

Polyclonal Antibody for studying RhoB. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, RhoB Antibody (CST #2098) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity RhoB Antibody detects endogenous levels of total RhoB protein. This antibody does not cross-react with RhoA or RhoC. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RhoB. Antibodies are purified using protein A and peptide affinity chromatography. Background Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1). RhoA, RhoB and RhoC are highly homologous, but appear to have divergent biological functions. Carboxy-terminal modifications and differences in subcellular localization allow these three proteins to respond to and act on distinct signaling molecules (2,3). RhoB functions in the regulation of cell shape, migration and adhesion (4). RhoB activity has also been shown to play a role in protein trafficking (5,6) and in CXCR2-mediated chemotaxis (6). Inhibition of RhoB activity downstream of PKCι influences the degree of invasion and migration by glioblastoma cells (7), and RhoB expression has a negative affect on tumor growth in ovarian cancer (8). Alternate Names Aplysia RAS-related homolog 6; ARH6; ARHB; h6; MST081; MSTP081; oncogene RHO H6; ras homolog family member B; ras homolog gene family, member B; Rho cDNA clone 6; Rho-related GTP-binding protein RhoB; RHOB; RHOH6 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 21 SOURCE: Rabbit