CST, 2105S, Phospho-Src (Tyr530) Antibody

Polyclonal Antibody for studying Src (Tyr530) phosphate. Validated for Western Blotting,Simple Western™,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Src (Tyr530) Antibody (CST #2105) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Phospho-Src (Tyr530) Antibody detects endogenous levels of Src only when phosphorylated at Tyr530. This antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr530 of human Src. Antibodies are purified by protein A and peptide affinity chromatography. Background The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr530 in the carboxy-terminal tail by Csk renders the enzyme less active (2). Alternate Names ASV; c-SRC; p60-Src; pp60c-src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; protooncogene SRC, Rous sarcoma; SRC; SRC proto-oncogene, non-receptor tyrosine kinase; SRC1; THC6; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog; v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 60 SOURCE: Rabbit