CST, 2106S, Hexokinase II Antibody

Polyclonal Antibody for studying Hexokinase 2. Validated for Western Blotting. Highly specific and rigorously validated in-house, Hexokinase II Antibody (CST #2106) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Hexokinase II Antibody detects endogenous levels of total hexokinase (isoform II) protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp341 of human hexokinase II protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III, and IV (glucokinase), have been identified. Hexokinases I, II, and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). Alternate Names DKFZp686M1669; hexokinase 2; Hexokinase type II; Hexokinase-2; hexokinase-2, muscle; Hexokinase-B; HK II; HK2; HKII; HXK2; Muscle form hexokinase Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 102 SOURCE: Rabbit