{"product_id":"cst-2112s","title":"CST,  2112S, Cycloheximide","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nCycloheximide is supplied as a lyophilized powder. For a 10 mg\/ml stock, carefully weigh out and reconstitute 50 mg in 5 ml DMSO or EtOH. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg\/ml for 4-24 hours. Soluble in DMSO, EtOH, or MeOH. Wear personal protective equipment. Do not empty product into drains. Do not handle until all safety precautions have been read and understood. Safety Information: Cycloheximide is suspected of causing genetic defects. It may cause adverse reproductive effects - such as birth defect, miscarriages, or infertility. Avoid contact during pregnancy and while nursing. If exposed or concerned, get medical advice. See Safety Data Sheet (SDS).\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze\/thaw cycles.\n\u003cb\u003eBackground\u003c\/b\u003e\nCycloheximide is a protein synthesis inhibitor in eukaryotes. Although its precise mechanism of action has yet to be fully elucidated, it has been shown to inhibit translation elongation through binding to the E-site of the 60S ribosomal unit and interfering with deacetylated tRNA (1-3). Although not all cell types are equally sensitive to the apoptosis-inducing effects of cycloheximide, it has been shown to induce cell death in T cells through a FADD-dependent mechanism (4). In addition, cycloheximide and Tumor Necrosis Factor possess a synergistic cytotoxicity (5,6), and consequently they are routinely used together to induce cell death. Investigators have demonstrated that cycloheximide blocks bortezomib-stimulated protein ubiquitination (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\napoptosis; bortezomib; CHX; cleaved parp; protein synthesis inhibitor; TNF; Tumor Necrosis Factor; ubiquitin","brand":"CST","offers":[{"title":"Default Title","offer_id":46800693166249,"sku":"2112S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2112s","provider":"Iright","version":"1.0","type":"link"}