{"product_id":"cst-2114t","title":"CST,  2114T, Rictor (53A2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Rictor. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Rictor (53A2) Rabbit Monoclonal Antibody (CST #2114) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRictor (53A2) Rabbit Monoclonal Antibody detects endogenous levels of total Rictor protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1681 of human Rictor protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian TOR (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, Sin1, and rictor (1). The mTOR-rictor complex phosphorylates Ser473 of Akt\/PKB (2). This phosphorylation is essential for full Akt\/PKB activation. Furthermore, an siRNA knockdown of rictor inhibits Ser473 phosphorylation in 3T3-L1 adipocytes (3). This complex has also been shown to phosphorylate the rapamycin-resistant mutants of S6K1, another effector of mTOR (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAVO3; AVO3 homolog; DKFZp686B11164; hAVO3; KIAA1999; mAVO3; MGC39830; PIA; pianissimo; Rapamycin-insensitive companion of mTOR; RICTOR; RICTR; RPTOR independent companion of MTOR complex 2; RPTOR independent companion of MTOR, complex 2; TORC2-specific protein AVO3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 200\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797655900329,"sku":"2114T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2114t","provider":"Iright","version":"1.0","type":"link"}