CST, 2125T, alpha-Tubulin (11H10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying tubulin alpha. Validated for WB,WB,IHC,IF,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, alpha-Tubulin (11H10) Rabbit Monoclonal Antibody (CST #2125) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Immunohistochemistry (Paraffin): 1:50 Immunofluorescence (Frozen): 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:400 Flow Cytometry (Fixed/Permeabilized): 1:50 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 77763 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity alpha-Tubulin (11H10) Rabbit Monoclonal Antibody detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster, Zebrafish, Bovine, Pig Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human α-tubulin protein. Background The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1). Alternate Names alpha tubulin; Alpha-tubulin ubiquitous; Detyrosinated tubulin alpha-1B chain; K-ALPHA-1; TBA1B; TUBA1B; tubulin alpha 1b; Tubulin alpha-1B chain; Tubulin alpha-ubiquitous chain; Tubulin K-alpha-1; tubulin, alpha 1b; tubulin, alpha, ubiquitous Specification REACTIVITY: H M R Mk Dm Z B Pg SENSITIVITY: Endogenous MW (kDa): 52 Source/Isotype: Rabbit IgG