CST, 2132S, Parkin Antibody

Polyclonal Antibody for studying Parkin. Validated for Western Blotting,Simple Western™. Highly specific and rigorously validated in-house, Parkin Antibody (CST #2132) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Parkin Antibody detects endogenous levels of total parkin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid 400 of human parkin. Antibodies are purified by protein A and peptide affinity chromatography. Background Parkin is a protein of 465 amino acids with an amino-terminal ubiquitin domain and a carboxy-terminal RING-box (1). In the case of autosomal recessive juvenile Parkinsonism (AR-JP), deletions have been found in the gene on chromosome 6 encoding the protein Parkin (2). Alternate Names AR-JP; E3 ubiquitin-protein ligase parkin; LPRS2; PARK2; parkin; parkin 2; parkin RBR E3 ubiquitin protein ligase; Parkin RBR E3 ubiquitin-protein ligase; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PRKN; PRKN2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 50 SOURCE: Rabbit