CST, 21792T, SS18 (D6I4Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SS18. Validated for WB,IP,IHC,ChIP,ChIP,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, SS18 (D6I4Z) Rabbit Monoclonal Antibody (CST #21792) is ready to ship. Product Usage Information For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:10000 Chromatin IP: 1:50 Chromatin IP-seq: 1:50 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 57558 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Chromatin IP, Chromatin IP-seq, CUT&RUN Specificity / Sensitivity SS18 (D6I4Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total SS18 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln394 of human SS18 protein. Background ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9). SS18 is a protein that has been shown to be a part of the SWI/SNF complex (10, 11). The SS18-SSX fusion proteins are a result of in-frame fusions that fuse the SS18 gene on chromosome 18 with X chromosome genes SSX1, SSX2, and to a lesser extent SSX4 (12). Human synovial sarcoma (SS) accounts for 8-10% of all soft tissue malignancies and 95% of these malignancies express the recurrent translocation of the SS18 gene on chromosome 18 (12). The N-terminal SNH domain (SYT N-terminal homology domain) of the SS18 protein interacts with SWI/SNF chromatin remodeling complexes via the N terminal region of BRM and BRG1 subunits (13). Studies of the SS18-SSX fusion in SS suggest that endogenous SS18 competes with the mutant SS18-SSX fusion for occupancy in the SWI/SNF complexes resulting in the displacement of SNF5 (BAF47) subunit. Displacement of the SNF5 subunit results in altered function of the SWI/SNF complex that leads to deregulated expression of genes such as Sox2 in SS (12). In addition, cytosolic SS18 isoforms also associate with F-actin in cytoskeletal organization (14). SS18 null mice do not develop beyond E9.5 and have defects in vascularization, cell migration, neural tube closure, and fusion within the embryonic-maternal membranes (14). Alternate Names MGC116875; Protein SSXT; Protein SYT; SS18; SS18 subunit of BAF chromatin remodeling complex; SS18, nBAF chromatin remodeling complex subunit; SSXT; Synovial sarcoma translocated to X chromosome protein; synovial sarcoma translocation, chromosome 18; synovial sarcoma, translocated to X chromosome; SYT Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): Iso1 60, Iso2 50 Source/Isotype: Rabbit IgG