{"product_id":"cst-2194s","title":"CST,  2194S, MG-132","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nMG-132 is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 1 mg in 210.3 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µM for 1-24 hours. Soluble in DMSO or EtOH.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 1 month to prevent loss of potency. Aliquot to avoid multiple freeze\/thaw cycles.\n\u003cb\u003eBackground\u003c\/b\u003e\nMG-132 is a potent proteasome and calpain inhibitor. Research studies have shown that MG-132 prevents degradation of the proteasome substrates SucLLVY-MCA and ZLLL-MCA with an IC of 850 nM and 100 nM, respectively, as well as inhibits casein-degrading activity of m-calpain with an IC of 1.25 µM (1). Investigators have demonstrated that MG-132 inhibits TNF-α-induced NF-κB activation and IL-8 release in A549 cells (2). Proteasome inhibition with MG-132 prevents degradation of short-lived proteins, which correlates with increased expression of HSP and ER chaperone proteins (3). MG-132 induces apoptosis in Hep G2 cells in a time- and dose-dependent manner (4). Proteasome inhibitors like MG-132 are important research tools for studying cellular degradation of the ubiquitin-proteasome pathway.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\napoptosis Hep G2; calpain inhibitor; casein-degrading activity; MG132; Phospo-cyclin; proteasome inhibitor; ubiquitin-proteasome; Z-Leu-Leu-Leu-CHO; Z-LLL-al","brand":"CST","offers":[{"title":"Default Title","offer_id":46800693231785,"sku":"2194S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2194s","provider":"Iright","version":"1.0","type":"link"}