CST, 2195S, Mnk1 (C4C1) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Mnk1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Mnk1 (C4C1) Rabbit Monoclonal Antibody (CST #2195) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Mnk1 (C4C1) Rabbit Monoclonal Antibody detects endogenous levels of total Mnk1 protein. Species Reactivity: Human, Mouse Source / Purification Mnk1 (C4C1) Rabbit mAb is produced by immunizing rabbits with a synthetic peptide corresponding to the sequence of human Mnk1. Background Mitogen-activated protein kinases (MAPKs) are activated by various extracellular signals and play crucial roles in regulating cell proliferation, differentiation, survival, and apoptosis (1). MAPK-interacting kinases (Mnks or MKNKs) are direct downstream substrates of MAPK and were first discovered independently by the work of Fukunaga and Hunter (2) and Waskiewicz and Cooper (3). There are 2 Mnks in human, termed Mnk1 and Mnk2. Both Mnks possess a MAPK-binding domain that allows them to bind to and then to be phosphorylated by Erk and p38. The phosphorylation in the T-loop of Mnks stimulates their kinase activity toward a substrate, eukaryotic initiation factor-4E (eIF4E) (2,3). eIF4E is a key component of the translational machinery mediating the initiation of translation, but how phosphorylation of eIF4E regulates translation initiation is still under investigation (4). Alternate Names MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK interacting serine/threonine kinase 1; MAPK signal-integrating kinase 1; MKNK1; MNK1 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG