CST, 2205S, Jagged2 (C83A8) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Jagged2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Jagged2 (C83A8) Rabbit Monoclonal Antibody (CST #2205) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Jagged2 (C83A8) Rabbit Monoclonal Antibody detects endogenous levels of total Jagged2 protein. This antibody does not cross-react with Jagged1. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1212 of human Jagged2. Background Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3, and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus, where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). Alternate Names hJ2; JAG2; jagged 2; jagged canonical Notch ligand 2; Jagged2; Protein jagged-2; SER2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 150 Source/Isotype: Rabbit IgG