CST, 2207T, Shh (C9C5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SHH. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Shh (C9C5) Rabbit Monoclonal Antibody (CST #2207) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Shh (C9C5) Rabbit Monoclonal Antibody detects the endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu53 of human Shh. Background Hedgehog proteins (Hh) are secreted signaling proteins that play many roles during animal development. Aberrant Hh signaling activity can be associated with numerous birth defects and uncontrolled Hh pathway activation is linked to the development of several types of cancers (1-2). The three identified vertebrate Hh genes are Sonic ( ), Indian ( ) and Desert ( ), all of which have distinct as well as overlapping roles (3-5). Hh proteins are synthesized as 45 kDa precursors that undergo auto-cleavage to generate a 19 kDa amino-terminal peptide (Hh-N) and a carboxy-terminal peptide (Hh-C). The Hh-N becomes covalently attached to a cholesterol molecule at its carboxy terminus and acetylated at its amino terminus. This doubly modified Hh-N peptide is released from cells and responsible for all known Hedgehog signaling activity (6). Alternate Names HHG-1; HHG1; HLP3; HPE3; MCOPCB5; SHH; Shh N-terminal processed signaling domains; Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; ShhN; ShhNC; ShhNp; SMMCI; sonic hedgehog homolog; sonic hedgehog homolog (Drosophila); Sonic hedgehog protein; Sonic hedgehog protein C-product; Sonic hedgehog protein N-product; sonic hedgehog signaling molecule; TPT; TPTPS Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 19, (45 precursor) Source/Isotype: Rabbit IgG