{"product_id":"cst-2222s","title":"CST,  2222S, Anisomycin","description":"Chemical Modulators for studying in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nAnisomycin is supplied as a lyophilized powder. For a 25 mg\/ml stock, reconstitute the 10 mg in 400 µl DMSO. Working concentrations and length of treatments vary depending on the desired effect, but it is typically used at 5-50 µg\/ml for 5-60 minutes. Soluble in DMSO or MeOH.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore lyophilized at -20C. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 1 month and store at -20C. Aliquot to avoid multiple freeze\/thaw cycles.\n\u003cb\u003eBackground\u003c\/b\u003e\nAnisomycin, an antibiotic produced by and , was originally described to inhibit protein-protein synthesis at the translational level (1). More recently, it is has been well characterized to strongly activate the stress kinases SAPK\/JNK and p38 MAPK, as well as p70\/85 S6 kinase in mammalian cells, which results in the rapid induction of immediate-early (IE) genes, such as c-fos, fosB, c-jun, JunB, and JunD (1). Investigators have demonstrated that anisomycin acts as a potent signaling agonist, synergizing with growth factors and phorbol esters to superinduce these IE genes (1,2). Research studies have demonstrated that anisomycin induces apoptosis in many cancer cell lines (3-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\napoptosis; c-fos; c-jun; fosB; JunD; p38 MAPK; p70\/85 S6 kinase; phorbol esters; phospho junB; SAPK\/JNK","brand":"CST","offers":[{"title":"Default Title","offer_id":46800693559465,"sku":"2222S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2222s","provider":"Iright","version":"1.0","type":"link"}