{"product_id":"cst-2251s","title":"CST,  2251S, FosB (5G4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying FosB. Validated for WB,IP,IHC,IF,F,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, FosB (5G4) Rabbit Monoclonal Antibody (CST #2251) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunohistochemistry (Paraffin): 1:50 - 1:200\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\nChromatin IP: 1:50\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 32170 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nFosB (5G4) Rabbit Monoclonal Antibody detects endogenous levels of total FosB protein (both FosB and FosB2 isoforms). This antibody does not cross-react with other Fos proteins, including c-fos, FRA1 and FRA2.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala140 of human FosB protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE\/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos\/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1\/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nactivator protein 1; AP-1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; FOSB; FosB proto-oncogene, AP-1 trancription factor subunit; FosB proto-oncogene, AP-1 transcription factor subunit; G0\/G1 switch regulatory protein 3; G0S3; GOS3; GOSB; MGC42291; oncogene FOS-B; Protein FosB\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 38 FosB2 48 FosB\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797662060713,"sku":"2251S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2251s","provider":"Iright","version":"1.0","type":"link"}