CST, 2262S, Naked1 Antibody

Polyclonal Antibody for studying Naked1. Validated for Western Blotting. Highly specific and rigorously validated in-house, Naked1 Antibody (CST #2262) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Naked1 Antibody detects endogenous levels of total Naked1 proteins. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln227 of human Naked1. Antibodies are purified by protein A and peptide affinity chromatography. Background Naked1 (Nkd1) and Naked2 (Nkd2) are homologs of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway which functions through a feedback mechanism (1,2). Both Drosophila and vertebrate Naked proteins contain a putative calcium-binding EF-hand motif, however, Drosophila Naked binds to zinc instead of calcium (3). Naked inhibits the canonical Wnt/β-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway (2,4). Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/β-catenin pathway (5). Naked2 is myristoylated and is required for sorting of TGF-α to the basolateral plasma membrane of polarized epithelial cells (6). Alternate Names Dvl-binding protein; hNkd; hNkd1; naked cuticle homolog 1; naked cuticle homolog 1 (Drosophila); naked cuticle-1; Naked-1; Naked1; NKD; NKD inhibitor of WNT signaling pathway 1; NKD1; NKD1, WNT signaling pathway inhibitor; Protein naked cuticle homolog 1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 59, 61 SOURCE: Rabbit