{"product_id":"cst-2305t","title":"CST,  2305T, JNK3 (55A8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying JNK3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, JNK3 (55A8) Rabbit Monoclonal Antibody (CST #2305) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nJNK3 (55A8) Rabbit Monoclonal Antibody detects endogenous levels of total JNK3 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human JNK3.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe stress-activated protein kinase\/Jun-amino-terminal kinase SAPK\/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4\/7. Upon activation, MKKs phosphorylate and activate the SAPK\/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4\/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK\/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK\/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nc-Jun N-terminal kinase 3; FLJ12099; FLJ33785; JNK3; JNK3 alpha protein kinase; JNK3A; MAP kinase 10; MAP kinase p49 3F12; MAPK 10; MAPK10; MGC50974; Mitogen-activated protein kinase 10; MK10; p493F12; p54bSAPK; PRKM10; SAPK1B; stress activated protein kinase beta; Stress-activated protein kinase 1b; Stress-activated protein kinase JNK3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 54\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797663502505,"sku":"2305T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2305t","provider":"Iright","version":"1.0","type":"link"}