CST, 23083SF, CDX2 (D11D10) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CDX2. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, CDX2 (D11D10) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #23083) is ready to ship. Product Usage Information This product is the carrier free version of product #12306. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 12306 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CDX2 (D11D10) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CDX2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CDX2 protein. Background CDX2, a homeobox domain-containing transcription factor, is a master regulator of the trophectoderm, the layer that gives rise to extra-embryonic tissues in mammalian development (1). CDX2 is also involved in intestinal development (2), and gain of expression or loss of expression has been associated with various human malignancies, such as Barrett's esophagus (3) and colorectal cancer (4,5). Mouse embryonic stem cells deficient in CDX2 display limited hematopoietic progenitor development and altered gene expression (6), pointing to a role for CDX2 in gene regulation. CDX2 is also implicated in the aberrant expression of genes in human AML cell lines (7). Alternate Names caudal type homeo box transcription factor 2; caudal type homeobox 2; caudal type homeobox transcription factor 2; Caudal-type homeobox protein 2; CDX-3; CDX2; CDX2/AS; CDX3; Homeobox protein CDX-2; homeobox protein miniCDX2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG