CST, 23111T, TIE1 (D2K2T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Tie1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, TIE1 (D2K2T) Rabbit Monoclonal Antibody (CST #23111) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity TIE1 (D2K2T) Rabbit Monoclonal Antibody recognizes endogenous levels of total TIE1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TIE1 protein. Background TIE1 belongs to the Tie family of receptor tyrosine kinases. TIE1 is structurally similar to its homolog TIE2, but differs from the latter in that it does not have a known ligand and is thus considered an orphan receptor (1). A key function of TIE1 is to modulate TIE2 signaling, via heterodimerization with TIE2 at the cell surface (2). The effects of TIE1-TIE2 interaction are context-dependent; heterodimerization can either promote or inhibit downstream TIE2 signaling depending on localized TIE2 levels (3-6). Research studies have shown TIE1 to be implicated in angiogenesis, vascular maturation, tissue remodeling, and inflammation. Increased expression of TIE1 has also been associated with cancer stemness and atherosclerosis formation (7, 8). Alternate Names JTK14; soluble TIE1 variant 1; soluble TIE1 variant 2; soluble TIE1 variant 3; soluble TIE1 variant 4; soluble TIE1 variant 5; TIE; TIE1; tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 1; tyrosine kinase with immunoglobulin like and EGF like domains 1; tyrosine kinase with immunoglobulin-like and EGF-like domains 1; Tyrosine-protein kinase receptor Tie-1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 135, 140 Source/Isotype: Rabbit IgG