CST, 2311S, Phospho-Synapsin (Ser9) Antibody

Polyclonal Antibody for studying Synapsin-1 (Ser9) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Synapsin (Ser9) Antibody (CST #2311) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Synapsin (Ser9) Antibody detects endogenous levels of synapsin only when phosphorylated at serine 9. This antibody is expected to recognize phosphorylated serine 9 of all synapsin isoforms. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 9 of human synapsin I. Antibodies are purified by protein A and peptide affinity chromatography. Background Synapsins, a group of at least five related members (synapsins Ia, Ib, IIa, IIb, and IIIa), are abundant brain proteins essential for regulating neurotransmitter release (1,2). All synapsins contain a short amino-terminal domain that is highly conserved and phosphorylated by PKA or CaM kinase I (1). Phosphorylation of the synapsin amino-terminal domain at Ser9 inhibits its binding to phospholipids and dissociates synapsins from synaptic vesicles (2). Alternate Names Brain protein 4.1; MRX50; SYN1; SYN1a; SYN1b; Synapsin; Synapsin I; synapsin Ib; Synapsin-1; SYNI Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 77 SOURCE: Rabbit