{"product_id":"cst-2319s","title":"CST,  2319S, NCK1 (15B9) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying NCK1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, NCK1 (15B9) Rabbit Monoclonal Antibody (CST #2319) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNCK1 (15B9) Rabbit Monoclonal Antibody detects the endogenous levels of total NCK1 protein. Based on homology it may also recognize NCK2.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly300 of human NCK1.\n\u003cb\u003eBackground\u003c\/b\u003e\nNCK adapter protein 1 (NCK1), also known as NCK or NCKα, is a broadly expressed oncogenic adapter protein consisting of three SH3 domains and one SH2 domain (1-3). NCK1 becomes phosphorylated upon activation of variety of cell surface receptors and is involved in actin cytoskeletal organization induced by many stimuli (4-6). NCK2, also known as NCKβ, a homolog of NCK1, has an overlapping expression pattern and redundant functions with NCK1 (7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK; NCK adaptor protein 1; NCK tyrosine kinase; Nck-1; NCK1; NCKalpha; non-catalytic region of tyrosine kinase; SH2\/SH3 adaptor protein NCK-alpha\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 47\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797664911529,"sku":"2319S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2319s","provider":"Iright","version":"1.0","type":"link"}