CST, 23207S, GAT1 Antibody

Polyclonal Antibody for studying GAT1. Validated for Western Blotting. Highly specific and rigorously validated in-house, GAT1 Antibody (CST #23207) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GAT1 Antibody recognizes endogenous levels of total GAT1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly583 of human GAT1 protein. Antibodies are purified by peptide affinity chromatography. Background The solute carrier 6 gene ( ), also known as the neurotransmitter-sodium-symporter family or Na+/Cl- -dependent transporter, encodes for proteins that regulate neurotransmitter (NTT) transport, including monoamine transmitters serotonin, dopamine, and norepinephrin (SERT), GABA transmitters (GAT1, GAT2, GAT3, and BGT1), and glycine transmitters (GLYT1 and GLYT2) (1). These proteins express ubiquitously in the brain and regulate the release and uptake of neurotransmitters in terminal clefts, in both neuronal and non-neuronal cells (2-4). Dysregulation of NTT-transporters is associated with neurological disease like epilepsy, schizophrenia, anxiety, bipolar disorder, and addictions to cocaine and methamphetamines (1). Inhibitors of NTT-transporters are widely used as drugs to treat disorders like depression (tricyclic antidepressants), and antiepileptic tiagabine (5). GAT1 is the only GABA transporter genetically studied in GAT1-KO mouse models where an accumulation of extracellular GABA decreased anxiety and depression-like behaviors (6-8). The lack or reduction of GAT1 diminished aggression in mice, and a condition known as hypoalgesia, where there is a decreased sensitivity to painful stimuli (8,9). GAT1 posttranslational modifications include phosphorylation at Tyr107 (IL1), and Tyr317 (IL3), and these mutations identify as the phospho-acceptor-sites, therefore regulating GAT1 (10,11). GABA trafficking is regulated by Tyr phosphorylation, and it has been shown that activation of adenosine A2A receptors in the hippocampus synaptosomes enhanced BAGA uptake by opposing a constitutive PKC-mediated downregulation of GAT1 (11-13). Alternate Names GABA transporter 1; GABATHG; GABATR; GABT1; GAT-1; GAT1; MAE; SC6A1; SLC6A1; Sodium- and chloride-dependent GABA transporter 1; solute carrier family 6 (neurotransmitter transporter, GABA), member 1; solute carrier family 6 (neurotransmitter transporter), member 1; Solute carrier family 6 member 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 65 SOURCE: Rabbit